Bacterial diversity and community composition from seasurface to subseafloor

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doi: 10.1038/ismej.2015.175
Author(s): Walsh, Emily A.; Kirkpatrick, John B.; Rutherford, Scott D.; Smith, David C.; Sogin, Mitchell; D'Hondt, Steven
Author Affiliation(s): Primary:
Forsyth Institute, Department of Microbiology, Cambridge, MA, United States
Other:
University of Rhode Island, United States
Roger Williams University, United States
Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, United States
Volume Title: ISME Journal
Source: The ISME Journal, 10(4), p.979-989. Publisher: Nature Publishing Group, London, United Kingdom. ISSN: 1751-7362
Note: In English. 46 refs.; illus., incl. sketch map
Summary: We investigated compositional relationships between bacterial communities in the water column and those in deep-sea sediment at three environmentally distinct Pacific sites (two in the Equatorial Pacific and one in the North Pacific Gyre). Through pyrosequencing of the v4-v6 hypervariable regions of the 16S ribosomal RNA gene, we characterized 450,104 pyrotags representing 29,814 operational taxonomic units (OTUs, 97% similarity). Hierarchical clustering and non-metric multidimensional scaling partition the samples into four broad groups, regardless of geographic location: a photic-zone community, a subphotic community, a shallow sedimentary community and a subseafloor sedimentary community (>1.5 meters below seafloor). Abundance-weighted community compositions of water-column samples exhibit a similar trend with depth at all sites, with successive epipelagic, mesopelagic, bathypelagic and abyssopelagic communities. Taxonomic richness is generally highest in the water-column O2 minimum zone and lowest in the subseafloor sediment. OTUs represented by abundant tags in the subseafloor sediment are often present but represented by few tags in the water column, and represented by moderately abundant tags in the shallow sediment. In contrast, OTUs represented by abundant tags in the water are generally absent from the subseafloor sediment. These results are consistent with (i) dispersal of marine sedimentary bacteria via the ocean, and (ii) selection of the subseafloor sedimentary community from within the community present in shallow sediment.
Year of Publication: 2015
Research Program: IODP Integrated Ocean Drilling Program
ODP Ocean Drilling Program
Key Words: 07 Marine Geology and Oceanography; Bacteria; Communities; East Pacific; Equatorial Pacific; Expedition 329; Integrated Ocean Drilling Program; Leg 201; Marine environment; Ocean Drilling Program; Pacific Ocean; South Pacific; Southeast Pacific; Species diversity
Coordinates: S455800 S235100 W1230900 W1660000
S120500 N035000 W0775500 W1103500
Record ID: 2019038142
Copyright Information: GeoRef, Copyright 2019 American Geosciences Institute.